研究方向：
1. 生物驱动（Bio-inspiration）：非粮资源营养特性及其生物转化工具的生物学基础研究
· 进行潜在饲料资源及原料的研究与利用，明确替代性饲料资源特性、应用限制因素，及其产生、消除的相关分子机制
· 新资源、新工具、新方法和碳氮转化循环新机制的发现
2. 生物整合（Bio-integration）：能量和蛋白资源的生物转化关键技术研究
· 基于生物催化、基因工程、发酵工程构建基于生物技术的生物转化利用技术
· 蛋白和能量转化的微生态路径改造
3. 生物智能（Bio-intelligence）
· 建立基于肠道组学快速分析技术和大数据分析技术的动物全生命周期营养健康管理体系
· 建立动物健康肠道靶点的评价和应用技术
代表性成果：
1．团队平台及技术储备
1.1 建立动物肠道菌群模拟分析技术平台
建立集智能粪菌分离、肠道菌群培养、肠道气体分析、TLC点样、全自动特定蛋白分析、短链脂肪酸分析的动物肠道菌群模拟分析系统，基于模拟的动物肠道菌群筛选研究新型饲料资源及其代谢机理，构建基于肠道菌群的饲料资源数据库。
1.2 揭示植物乳杆菌通过“肠-肝”轴缓解高脂饮食所导致的脂质代谢异常
通过高脂饮食构建肥胖小鼠模型，植物乳杆菌通过调节肠道微生物为起点，增加菌群多样性与丁酸产生菌的相对丰度，通过肠道内容物代谢组、肝脏代谢组以及血清代谢组研究分析，共同揭示了植物乳杆菌通过“肠-肝”轴调制甘油磷脂代谢缓解脂质代谢异常。
1.3 微生物菌株及资源库
目前已储备饲用菌资源千余株，含乳酸菌、芽孢杆菌、酵母菌等饲用微生物，以及降解纤维、羽毛、氨氮、粪臭素和霉菌毒素等功能微生物百余株。同时利用基于Biolog自动微生物鉴定系统对重点菌种菌株进行精确鉴定，建立各菌株的代谢谱图库。
1.4 新型替代玉米豆粕饲料原料研究
建立饲料原料生物预处理技术，研制新型饲料原料6种，降低饲料中有毒有害成分40%-90%，营养价值提升20%以上，研制的新型原料可替代玉米豆粕10%以上。
1.5 发酵工艺技术及应用技术研究
基于高效降解大豆抗原蛋白的解淀粉芽孢杆菌，建立豆粕混菌固态发酵工艺；以筛选获得高效降解硫甙的嗜酸乳杆菌为基础，建立菜籽粕混菌厌氧固态发酵工艺；建立花生粕菌酶协同发酵中试工艺；筛选确立了棕榈仁粕菌酶协同发酵中试工艺；建立蔬菜尾菜——西兰花茎叶混菌厌氧固态发酵工艺；建立杏鲍菇菌糠发酵工艺；建立米糠粕固态发酵小试工艺；羽毛粉固态发酵工艺；固态发酵产品提高肉鸡日增重和饲料转化率，免疫力以及肠道紧密连接性，降低腹脂率，此外，改善肠道菌群结构。
2． 发明专利
刘国华, 吴正可，常文环. 蔡辉益. 一种嗜酸乳杆菌及其筛选方法和应用、一种菌剂. ZL 2017114005343.
刘国华，郑爱娟，王永东. 一种西兰花茎叶发酵饲料及其制备方法. ZL 2014101479367.
牛灿芳，杨培龙，姚斌，李阳阳，杜永凯，罗会颖，黄火清，王亚茹. Phytase variants YeAPPA having improved gastric protein resistance and acid resistance, and increased catalytic efficiency，2021年，美国US 11,155,793 B2
牛灿芳，杨培龙，姚斌，李阳阳，杜永凯，罗会颖，黄火清，王亚茹. Phytase variants YkAPPA having improved pepsin resistance and increased catalytic efficiency，2021年，美国US 11,028,377 B2
3. 代表性论文与专著发表
1. Chong Li, Shuzhen Li, Jinmei Liu, Huiyi Cai, Guohua Liu, Xuejuan Deng, Wenhuan Chang. Escherichia coli O88 Induces Intestinal Damage and Inflammatory Response through the Oxidative Phosphorylation and Ribosome Pathway in Pekin Ducks. Frontiers in Cellular and Infection Microbiology. 2022.
2. Chong Li, Huiyi Cai, Shuzhen Li, Xuejuan Deng, Wayne L.Bryden, Aijuan Zheng. Comparing the potential of Bacillus amyloliquefaciens CGMCC18230 with antimicrobial growth promoters for growth performance, bone development, expression of phosphorus transporters, and excreta microbiome in broiler chickens. Poultry Science. 2022.
3. Zhengke Wu, Kexin Yang, Anrong Zhang, Wenhuan Chang, Aijuan Zheng, Zhimin Chen, Huiyi Cai, Guohua Liu*. Effects of Lactobacillus acidophilus on the growth performance, immune response, and intestinal barrier function of broiler chickens challenged with Escherichia coli O157. Poultry Science. 2021
4. Zhengke Wu, Jiao Liu, Jiang Chen, Shoaib Ahmed Pirzado, Yang Li, Huiyi Cai and Guohua Liu*. Effects of Fermentation on Standardized Ileal Digestibility of Amino Acids and Apparent Metabolizable Energy in Rapeseed Meal Fed to Broiler Chickens. Animals. 2020.
5. Zhengke Wu, Jiang Chen, Shoaib Ahmed Pirzado, Tesfay Hagos Haile, Huiyi Cai, Guohua Liu. The effect of fermented and raw rapeseed meal on the growth performance, immune status and intestinal morphology of broiler chickens. Journal of Animal Physiology and Animal Nutrition. 2021.
6. Yang Li, Baozhu Guo, Chong Li, WeiweiWang, Zhengke Wu, Guohua Liu and Huiyi Cai*. Isolation of a Highly Efficient antigenic- Protein- Degrading Bacillus amyloliquefaciens and Assessment of Its Safety. Animals. 2020.
7. Jiang Chen, Huakang Wang, Zhengke Wu, Hongxia Gu, Chong Li, Shaolong Wang, Guohua Liu. Effects of 5-aminolevulinic acid on the inflammatory responses and antioxidative capacity in broiler chickens challenged with lipopolysaccharide. Animal. 2022
8. 《中国生物饲料研究进展与发展趋势（2020）》，蔡辉益主编，2020，中国农业出版社。
9. Hongying Cai, Zhiguo Wen, Xiumei Li, Kun Meng, Peilong Yang, Lactobacillus plantarum FRT10 alleviated high-fat diet-induced obesity in mice through regulating PPARα signal pathway and gut microbiota. Applied Microbiology and Biotechnology, 2020, 104(13)5959-5972.
10. Hongying Cai, Zhiguo Wen, Kun Meng, Peilong Yang. Metabolomic signatures for liver tissue and cecum contents in high-fat diet-induced obese mice based on UHPLC-Q-TOF/MS. Nutrition & Metabolism, 2021, 18(1):69.
11. Hongying Cai, Zhiguo Wen, Xin Xu, Jiaxin Wang, Xuan Li, Kun Meng, Serum Metabolomics Analysis for Biomarker of Lactobacillus plantarum FRT4 in High-fat Diet-induced Obese Mice. Foods, 2022,11(2):184.
12. Hongying Cai, Zhiguo Wen, Kun Meng, Peilong Yang. Lactobacillus plantarum FRT4 alleviated obesity by modulating gut microbiota and liver metabolome in high-fat diet-induced obese mice. Food & Nutrition Research, 2022, 66:7974.
13. Hongying Cai, Daojie Li, Liye Song, Xin Xu, Yunsheng Han, Kun Meng, Zhiguo Wen, Peilong Yang. Metabolomic characteristics of liver and cecum contents in high-fat-diet-induced obese mice intervened with Lactobacillus plantarum FRT10. 2022, 11(16):2491.
14. Han Yunsheng, Zhan Tengfei, Zhao Qingyu, Tang Chaohua, Zhang Kai., Han Yanming, Zhang Junmin*. Effects of mixed organic acids and medium chain fatty acids as antibiotic alternatives on the performance, serum immunity, and intestinal health of weaned piglets orally challenged with Escherichia coli K88. Animal Feed Science and Technology, 2020, 269. doi: 10.1016/j.anifeedsci.2020.114617.
15. Han Yunsheng, Zhao Qingyu, Tang Chaohua, Li Ying, Zhang Kai, Li Fadi*, Zhang Junmin*. Butyrate mitigates weanling piglets from lipopolysaccharide-induced colitis by regulating microbiota and energy metabolism of the gut–liver axis. Frontiers in Microbiology, 2020. doi:10.3389/fmicb.2020.588666.
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